[CD15 focus score for diagnostics of periprosthetic joint infections : Neutrophilic granulocytes quantification mode and the development of morphometric software (CD15 quantifier)].
Identifieur interne : 000196 ( PubMed/Checkpoint ); précédent : 000195; suivant : 000197[CD15 focus score for diagnostics of periprosthetic joint infections : Neutrophilic granulocytes quantification mode and the development of morphometric software (CD15 quantifier)].
Auteurs : B. Kölbel [Allemagne] ; S. Wienert ; J. Dimitriadis ; D. Kendoff ; T. Gehrke ; M. Huber ; L. Frommelt ; A. Tiemann ; K. Saeger ; V. KrennSource :
- Zeitschrift fur Rheumatologie [ 1435-1250 ] ; 2015.
English descriptors
- KwdEn :
- Aged, Aged, 80 and over, Antigens, CD15, Bacterial Infections (blood), Bacterial Infections (diagnosis), Bacterial Infections (immunology), Biomarkers (blood), Diagnosis, Computer-Assisted (methods), Fucosyltransferases, Humans, Male, Middle Aged, Neutrophils (immunology), Prosthesis-Related Infections (blood), Prosthesis-Related Infections (diagnosis), Prosthesis-Related Infections (immunology), Reproducibility of Results, Sensitivity and Specificity, Software.
- MESH :
- chemical , blood : Biomarkers.
- chemical : Antigens, CD15, Fucosyltransferases.
- blood : Bacterial Infections, Prosthesis-Related Infections.
- diagnosis : Bacterial Infections, Prosthesis-Related Infections.
- immunology : Bacterial Infections, Neutrophils, Prosthesis-Related Infections.
- methods : Diagnosis, Computer-Assisted.
- Aged, Aged, 80 and over, Humans, Male, Middle Aged, Reproducibility of Results, Sensitivity and Specificity, Software.
Abstract
The aim of this project was to devise a quantification method for neutrophils within a single focal point through the development of a CD15 focus score which enables bacterial infections in synovial-like interface membranes (SLIM) to be diagnosed.
DOI: 10.1007/s00393-015-1571-8
PubMed: 25869074
Affiliations:
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Wienert</name></author><author><name sortKey="Dimitriadis, J" sort="Dimitriadis, J" uniqKey="Dimitriadis J" first="J" last="Dimitriadis">J. Dimitriadis</name></author><author><name sortKey="Kendoff, D" sort="Kendoff, D" uniqKey="Kendoff D" first="D" last="Kendoff">D. Kendoff</name></author><author><name sortKey="Gehrke, T" sort="Gehrke, T" uniqKey="Gehrke T" first="T" last="Gehrke">T. Gehrke</name></author><author><name sortKey="Huber, M" sort="Huber, M" uniqKey="Huber M" first="M" last="Huber">M. Huber</name></author><author><name sortKey="Frommelt, L" sort="Frommelt, L" uniqKey="Frommelt L" first="L" last="Frommelt">L. Frommelt</name></author><author><name sortKey="Tiemann, A" sort="Tiemann, A" uniqKey="Tiemann A" first="A" last="Tiemann">A. Tiemann</name></author><author><name sortKey="Saeger, K" sort="Saeger, K" uniqKey="Saeger K" first="K" last="Saeger">K. Saeger</name></author><author><name sortKey="Krenn, V" sort="Krenn, V" uniqKey="Krenn V" first="V" last="Krenn">V. 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Kölbel</name><affiliation wicri:level="1"><nlm:affiliation>Zentrum für Histologie, Zytologie und Molekulare Diagnostik, Max-Planck-Str. 5, 54296, Trier, Deutschland.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Zentrum für Histologie, Zytologie und Molekulare Diagnostik, Max-Planck-Str. 5, 54296, Trier</wicri:regionArea><wicri:noRegion>54296, Trier</wicri:noRegion><wicri:noRegion>54296, Trier</wicri:noRegion><wicri:noRegion>Trier</wicri:noRegion></affiliation></author><author><name sortKey="Wienert, S" sort="Wienert, S" uniqKey="Wienert S" first="S" last="Wienert">S. Wienert</name></author><author><name sortKey="Dimitriadis, J" sort="Dimitriadis, J" uniqKey="Dimitriadis J" first="J" last="Dimitriadis">J. Dimitriadis</name></author><author><name sortKey="Kendoff, D" sort="Kendoff, D" uniqKey="Kendoff D" first="D" last="Kendoff">D. Kendoff</name></author><author><name sortKey="Gehrke, T" sort="Gehrke, T" uniqKey="Gehrke T" first="T" last="Gehrke">T. Gehrke</name></author><author><name sortKey="Huber, M" sort="Huber, M" uniqKey="Huber M" first="M" last="Huber">M. Huber</name></author><author><name sortKey="Frommelt, L" sort="Frommelt, L" uniqKey="Frommelt L" first="L" last="Frommelt">L. Frommelt</name></author><author><name sortKey="Tiemann, A" sort="Tiemann, A" uniqKey="Tiemann A" first="A" last="Tiemann">A. Tiemann</name></author><author><name sortKey="Saeger, K" sort="Saeger, K" uniqKey="Saeger K" first="K" last="Saeger">K. Saeger</name></author><author><name sortKey="Krenn, V" sort="Krenn, V" uniqKey="Krenn V" first="V" last="Krenn">V. Krenn</name></author></analytic><series><title level="j">Zeitschrift fur Rheumatologie</title><idno type="eISSN">1435-1250</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aged</term><term>Aged, 80 and over</term><term>Antigens, CD15</term><term>Bacterial Infections (blood)</term><term>Bacterial Infections (diagnosis)</term><term>Bacterial Infections (immunology)</term><term>Biomarkers (blood)</term><term>Diagnosis, Computer-Assisted (methods)</term><term>Fucosyltransferases</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Neutrophils (immunology)</term><term>Prosthesis-Related Infections (blood)</term><term>Prosthesis-Related Infections (diagnosis)</term><term>Prosthesis-Related Infections (immunology)</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term><term>Software</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Biomarkers</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antigens, CD15</term><term>Fucosyltransferases</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Bacterial Infections</term><term>Prosthesis-Related Infections</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Bacterial Infections</term><term>Prosthesis-Related Infections</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Bacterial Infections</term><term>Neutrophils</term><term>Prosthesis-Related Infections</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Diagnosis, Computer-Assisted</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Aged</term><term>Aged, 80 and over</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term><term>Software</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The aim of this project was to devise a quantification method for neutrophils within a single focal point through the development of a CD15 focus score which enables bacterial infections in synovial-like interface membranes (SLIM) to be diagnosed.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25869074</PMID><DateCreated><Year>2015</Year><Month>09</Month><Day>05</Day></DateCreated><DateCompleted><Year>2016</Year><Month>06</Month><Day>01</Day></DateCompleted><DateRevised><Year>2015</Year><Month>09</Month><Day>05</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1435-1250</ISSN><JournalIssue CitedMedium="Internet"><Volume>74</Volume><Issue>7</Issue><PubDate><Year>2015</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Zeitschrift fur Rheumatologie</Title><ISOAbbreviation>Z Rheumatol</ISOAbbreviation></Journal><ArticleTitle>[CD15 focus score for diagnostics of periprosthetic joint infections : Neutrophilic granulocytes quantification mode and the development of morphometric software (CD15 quantifier)].</ArticleTitle><Pagination><MedlinePgn>622-30</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00393-015-1571-8</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">The aim of this project was to devise a quantification method for neutrophils within a single focal point through the development of a CD15 focus score which enables bacterial infections in synovial-like interface membranes (SLIM) to be diagnosed.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">In this study a histopathological classification of 91 SLIM removed during revision surgery from the hips (n = 59) and knees (n = 32) was performed. Neutrophils were identified immunohistochemically by means of a CD15-specific monoclonal antibody. The quantitative evaluation of CD15-positive neutrophils (CD15Ne) used the principle of maximum focal infiltration (focus) together with an assessment of a single focal point (0.3 mm(2)). This immunohistochemical approach made it possible to develop the CD15 quantifier software, which automatically quantifies CD15Ne.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The SLIM cases with positive microbiological findings (n = 47) had significantly (p < 0.001, Mann-Whitney U-test) more CD15Ne/focal point than cases with negative microbiological findings (n = 44). A count of 50 CD15Ne/focal point was identified as the optimum threshold when diagnosing periprosthetic joint infections (PJI) using the CD15 focus score. If the microbiological findings are used as a gold standard, the diagnostic sensitivity is 0.83, and the specificity is 0.864 with a positive predictive value (PPV) of 0.87, a negative predictive value (NPV) of 0.83, an accuracy of 0.846 and an area under the curve (AUC) of 0.878. The evaluation of findings for the preparations using the CD15 quantifier software (n = 31) deviated by an average of 12 cells from the histopathological evaluation findings (CD15 focus score). Above a cell count of 62, the CD15-quantifier needs on average 32 s less than the pathologist.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The immunohistochemical CD15 focus score has a high diagnostic value and allowed the development of the CD15 quantifier software. This provides an automated procedure, which shortens the mentally tiring and time-consuming process of microscopic cell counting and thus makes a contribution towards the standardization of tools for diagnosing PJI.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kölbel</LastName><ForeName>B</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Zentrum für Histologie, Zytologie und Molekulare Diagnostik, Max-Planck-Str. 5, 54296, Trier, Deutschland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wienert</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Dimitriadis</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Kendoff</LastName><ForeName>D</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Gehrke</LastName><ForeName>T</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Huber</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Frommelt</LastName><ForeName>L</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Tiemann</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Saeger</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Krenn</LastName><ForeName>V</ForeName><Initials>V</Initials></Author></AuthorList><Language>ger</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><VernacularTitle>CD15-Fokus-Score zur Diagnostik der periprothetischen Gelenkinfektion : Neutrophile-Granulozyten-Quantifizierungs-Modus und Entwicklung einer morphometrischen Software ("CD15-Quantifier").</VernacularTitle></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Z Rheumatol</MedlineTA><NlmUniqueID>0414162</NlmUniqueID><ISSNLinking>0340-1855</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016256">Antigens, CD15</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015415">Biomarkers</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.-</RegistryNumber><NameOfSubstance UI="C066693">FUT4 protein, human</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.-</RegistryNumber><NameOfSubstance UI="D005647">Fucosyltransferases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000368" MajorTopicYN="N">Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000369" MajorTopicYN="N">Aged, 80 and over</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016256" MajorTopicYN="N">Antigens, CD15</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001424" MajorTopicYN="N">Bacterial Infections</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015415" MajorTopicYN="N">Biomarkers</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003936" MajorTopicYN="N">Diagnosis, Computer-Assisted</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005647" MajorTopicYN="N">Fucosyltransferases</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009504" MajorTopicYN="N">Neutrophils</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016459" MajorTopicYN="N">Prosthesis-Related Infections</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012984" MajorTopicYN="N">Software</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>4</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>4</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>6</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25869074</ArticleId><ArticleId IdType="doi">10.1007/s00393-015-1571-8</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country></list><tree><noCountry><name sortKey="Dimitriadis, J" sort="Dimitriadis, J" uniqKey="Dimitriadis J" first="J" last="Dimitriadis">J. Dimitriadis</name><name sortKey="Frommelt, L" sort="Frommelt, L" uniqKey="Frommelt L" first="L" last="Frommelt">L. Frommelt</name><name sortKey="Gehrke, T" sort="Gehrke, T" uniqKey="Gehrke T" first="T" last="Gehrke">T. Gehrke</name><name sortKey="Huber, M" sort="Huber, M" uniqKey="Huber M" first="M" last="Huber">M. Huber</name><name sortKey="Kendoff, D" sort="Kendoff, D" uniqKey="Kendoff D" first="D" last="Kendoff">D. Kendoff</name><name sortKey="Krenn, V" sort="Krenn, V" uniqKey="Krenn V" first="V" last="Krenn">V. Krenn</name><name sortKey="Saeger, K" sort="Saeger, K" uniqKey="Saeger K" first="K" last="Saeger">K. Saeger</name><name sortKey="Tiemann, A" sort="Tiemann, A" uniqKey="Tiemann A" first="A" last="Tiemann">A. Tiemann</name><name sortKey="Wienert, S" sort="Wienert, S" uniqKey="Wienert S" first="S" last="Wienert">S. Wienert</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Kolbel, B" sort="Kolbel, B" uniqKey="Kolbel B" first="B" last="Kölbel">B. 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